WEEK 08: Synthetic Minimal Cells
This journal documents the experiment of cell-free protein synthesis reactions: synthesis of GFP or FIAsH protein.
This topic is supported by David Sun Kong (MIT Media Lab) and Kate Adamala (University of Minnesota)
I. INTRO
GFP is green fluorescent protein – you can go learn more on Wikipedia. Mature GFP is fluorescent on it’s own – the fluorophore is made of amino acids from the GFP protein itself.
FIAsH is a very short protein, not fluorescent on its own. The fluorescence comes from the ligand, a small molecule that’s bound to the FIAsH peptide. The ligand’s full name is fluorescein arsenical helix binder bis-EDT adduct, FIAsH-EDT2.
II. MATH PREPARATION
We calculate final volumes and concentrations of our expression reaction in final reaction volume at 15uL total.
We then carefully setup all the reactions.
*To be honest the calculation part of this lab experiment is still obscure to me, I will update later after more research.
